While doing an experiment recently, I found that I was doing a lot of guesswork to figure out how much I should dilute resuspended colonies to get nicely spaced single colonies for viable counts. Since I was doing the simple calculations a lot, I decided to put together a worksheet to do it for me.
I also added a panel that does the calculation the other direction –from # of colonies on a plate back to the original # of cells in the original culture.
Trivial? Yes.
Useful? Hopefully.
Accurate? Probably.
The RADIO 